Catabolism of very low density lipoproteins in experimental nephrosis.
نویسندگان
چکیده
منابع مشابه
Catabolism of very low density lipoproteins in experimental nephrosis.
The effects of experimental nephrosis in rats, produced by puromycin aminonucleoside, include an elevation of plasma levels of all lipoprotein density classes and the appearance of high density lipoprotein (HDL) rich in apoprotein (apo) A-I and deficient in apo A-IV and apo E. The hyperlipoproteinemia is associated with an increase in hepatic synthesis of lipoproteins. The possible role of decr...
متن کاملvery-low-density lipoproteins
1. Human total HDL (high-density lipoprotein), HDL2 and HDL3 were labelled in vitro by incubation with lipoproteindeficient serum (LPDS) which contained either [3H]cholesteryl oleate or [14C]cholesterol under different conditions. The lipoproteins were then subfractionated by heparin-Sepharose column chromatography, and three subfractions (A, B and C) were successively eluted from each preparat...
متن کاملCharacterization and catabolism of rat very high density lipoproteins.
A previously unrecognized lipoprotein of very high density was isolated from rat serum. During zonal ultracentrifugation of whole serum or of fractions from Sepharose 4B chromatography, a peak comigrating with a peak of cholesterol was found between the typical high density lipoproteins and the residual serum proteins. Centrifugation of chylomicrons, very low density lipoproteins, and high dens...
متن کاملCatabolism of human very low density lipoproteins in vitro: a fluorescent phospholipid method for monitoring lipolysis.
The catabolism of human plasma very low density lipoproteins (VLDL) by purified bovine milk lipoprotein lipase has been measured in vitro using a fluorescent phospholipid as a method to monitor lipolysis. Dansyl phosphatidylethanolamine (DPE) was incorporated into VLDL to form DPE-VLDL, and the rate of catabolism was followed by measuring the increase in fluorescence at 490 nm after the additio...
متن کاملDansyl Phosphatidylethanolamine-labeled Very Low Density Lipoproteins
The fluorescent phospholipid dansyl phosphatidylethanolamine (DPE) (dansyl, 5-dimethylaminonaphthalene-1-sulfonyl) was incorporated into very low density lipoproteins (VLDL) to form DPE-VLDL. The addition of milk lipoprotein lipase to DPE-VLDL in the presence of albumin resulted in a greater than %fold fluorescence increase and a 20 nm blue shift in the wavelength of the emission maxima of t...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1984
ISSN: 0021-9738
DOI: 10.1172/jci111548